Oxidative stress has been implicated in pathology of many diseases such as inflammatory conditions, cancer, diabetes and aging. Hence an attempt was made to evaluate free radical scavenging activity, cytotoxic activity and polyphenolic content of methanolic extract of Calotropis procera flowers. Materials and methods: The free radical scavenging activity was estimated using in vitro models like 1,1,-diphenyl-2- picryl hydrazyl (DPPH) and hydroxyl radical. Cytotoxicity was analysed following MTT assay using Hep2 and Vero cell lines and polyphenols were estimated using standard methods. Two way ANOVA was used for statistical analyses. Result: The methanol extract of C.procera at 500µg/ml showed better scavenging activity in hydroxyl radical scavenging (66.03 %) which was followed by DPPH assay (50.82 %). The extract has 100 % cytotoxicity on Hep2 cell lines. Flavonoids were found to greater than phenols. Conclusion: It was suggested that the flowers of C.procera possess in vitro antioxidant and cytotoxic activity and having much therapeutic value.
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